Question

How does chloramphenicol inhibit protein synthesis? i have to prepare a 5 mark answer....

Answer 1

Chloramphenicol inhibits protein synthesis during elongation.
It's important to know that it inhibits during elongation, because the result are incomplete proteins.
Chloramphenicol binds to the 50s subunit ribosome during elongation, leading to shortened, incomplete proteins.
It stops the peptidyl transferase action.

Answer 2

Does it only stop protein synthesis in prokaryotes? or does it work on eukaryotes also? i.e. does it bind to the 60S subunit? @blackilluminati

Answer 3

i alsi have the same ques...does it occur in eukaryotic as well?

Answer 4

There's a vast difference between the prokaryotes and the eukaryotes ribosome structures.
50s ribosome for the prokaryotes and 60s ribosomes for the eukaryotes.

Answer 5

lol im well aware of that... hence why i asked if the inhibition occurs also in eukaryotes?

Answer 6

till now i came across prokaryotic translation inhibition only...:/

Answer 7

only in prokaryotes

Answer 8

thanks.:D..i still need to find the mechanism by which it inhibits....

Answer 9

do you have a reference for that?

Answer 10

not really...google...pubmed would be my reference...do u have any?

Answer 11

i was talking bout the inhibition only observed in prokaryotes. i sawa wiki page aout it though, you can check their references

Answer 12

aahh...yes..dat will do....thnx:D