You can find the non-pathogenic bacterium Micrococcus luteus in the soil and on human skin. The genome of this bacterium consists of double-stranded DNA with a base composition of 15% adenine. Predict which one of the following values represents the percentage of guanine.
Remember that the composition of each must equal 1. Also, remember that A pairs with T, and G with C, by Chargaff's rule, meaning that it should be a 1:1 ratio. So, if you have 15% A, you must also have 15% G, yes? Together, that equals 30%. Now, if you have 30% A-G pairs, how much of G-C, base pairs do you have together?
Sorry, I mean 15% A and 15% \(\sf \color{red}{T}\)
30% \(\sf \color{red}{A-T}\) pair*
So you would have 70% G-C pairs? since it must add up to be 100%
Yes, 70% C-G bonds, but you're trying to find \(\sf \color{red}{ONLY}\) guanine. So, 70% is not the correct answer. Instead, divide it by 2. Since remember, by chargaff's rule, it should be a 1:1 ratio of each.
AHA! 35%
Yes, 35% of guanine. And as you can see, you already checked yourself above, 35+35+15+15 = 100
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