Question

When monitoring the concentration of a protein by spectrophotometry an absorbance value of 0,6 was measured for 2ml of a 0,8mM aqueous solutio of the protein. After dilution with water, an absorbance of 0,3 was measured. What is the new concentration of the protein solution?

Answer 1

I was thinking it's similar to what we did, but what dose absorbance mean?

Answer 2

you're using the beer-lambert law, \(Abs=\epsilon*C*l\); which states that \(absorbance\) is \(\propto\) to \(concentration\).

Assuming \(\epsilon\) and path-length, \(l\), didn't change.

\(\dfrac{Abs_2}{C_2}=\dfrac{Abs_1}{C_1}\)

Answer 3

awww, thank you a lot!