Liquid Chromatography: In the step gradient separation, four separate fractions were collected. How were these related to the polarities of the column and of the eluting solvent?

Question

anonymous · Answer

For this particular lab, the column consisted of C18, a nonpolar substance, while the eluting solvent was 18% Isopropyl alcohol, a polar substance. this is all i have so far

dan815 · Answer

what was the difference in polarity of he substances

dan815 · Answer

palmer why are u here in chem land -.-

anonymous · Answer

no clue. all we were told was polar or nonpolar in the ingredietns ofr Koolaid (the solution to separate)

whpalmer4 · Answer

@dan815 this is the math forum...why is the chemistry question here is the real question...had it been tucked safely away in chemistry land, I might never have been forced to confront my lack of knowledge about LC :-)

dan815 · Answer

hahaha me too T_T

dan815 · Answer

i just googled/youtubed liquid chromatography

anonymous · Answer

I put it in both because some of the steps in the lab are math-esque

whpalmer4 · Answer

I told her that you know everything that I don't, so we're counting on you...but no pressure, eh? :-)

whpalmer4 · Answer

if we can't solve your problem, maybe we can at least entertain you for a while :-)

dan815 · Answer

is this column chromatograpy lab?

anonymous · Answer

its called Liquid Chromatograpy by Flinn Scientific

anonymous · Answer

it might be and we just did it on a smaller scale

dan815 · Answer

http://www.youtube.com/watch?v=TgJS7iDSXOA is that it?

anonymous · Answer

@primeralph

anonymous · Answer

that is the basic idea......

primeralph · Answer

Polar molecules move faster in chromatographics processes.

anonymous · Answer

alright.... how is that related to the column and the solvent?

primeralph · Answer

The question doesn't say much.
In short, the non-polar chemicals sit lower since they experience no force, while the polar molecules go higher in the column; if the solvent is polar too.

anonymous · Answer

we used Koolaid. 18% Isopropyl alcohol as the solvent and C18 as the column that the koolaid went through

anonymous · Answer

we performed the lab using a little syringe, thing with a small column on the end...

dan815 · Answer

gg