can someone explain to me why most of the mechanisms for recombinase attack by the 3'-OH, but the tyrosine recombinase attack by the 5'-OH?

Question

anonymous · Answer

@wolfe8

anonymous · Answer

what does that mean?

anonymous · Answer

did he address this answer in a previous post?

anonymous · Answer

sorry I'm new

anonymous · Answer

I mentioned him, so he can can help when he's online :P

anonymous · Answer

o i c, tyvm for the help

anonymous · Answer

No Prob, anytime.

wolfe8 · Answer

Oh boy. Sorry but I only did biology a little in high school. I don't think I learned about the DNA much. Did you mean attack or attach? 


@aaronq

anonymous · Answer

well this is graduate level biology lol. This is the mechanism of attachment of the recombinant to a target DNA strand. The recombinase enzyme cleaves the target gene and rejoins the 2 different segments. I was just wondering why the tyrosine was different compared to all the other type of recombinase mechanism

wolfe8 · Answer

Ah. Well you'll want to wait for someone else then. Good luck.

anonymous · Answer

alright thanks for ur help

anonymous · Answer

if it does not get answered soon I will just ask my professor lol. just was wondering if anyone knew it already

anonymous · Answer

@shrutipande9

shrutipande9 · Answer

heya sunshao...i have studied about this a lil...but i really have no idea about this...i m still trying to find an ans...if i get 1 ill post it here....