How can I measure when an Enzyme denatures?

Question

abb0t · Answer

I only am familiar with one of these methods, it is called dual polarizatio interferometry and possibly proteolysis.

anonymous · Answer

Is there an easier way to do this? Like by measuring the amount of Oxygen or the Pressure in the test tube?

abb0t · Answer

Can you maybe be a bit more specific as to what you're asking? I feel like the question you're asking is not properly written.

anonymous · Answer

Dang. My Biology teacher told me to create a lab using enzymes and this was the most original one I could create. I will be using a Poly ProBath to test out 5 test tubes with Catalase and Hydrogen Peroxide. Each one of these test tubes will be put in the bath, and each time the temperature will be different for each test tube (from 36C - 41C).

abb0t · Answer

Well, I am not familiar with the enzyme catalase. But it says on wikipedia that it functions in catalysis of peroxides in organisms. You can always measure the activity of the enzyme, yes at different temperatures. Then, measure the enzyme activity. obviously, if you get a low enzymy activity, it might tell you the infromation you're looking for.

Yes. at different temperatures, proteins will denature, going from quarternary/ tertiary to secondary even primary structures if exposed to TOO much heat.

anonymous · Answer

Alright that sounds good. Thanks for the help!