Question

What would you hypothesize for an investigation to find the effect of temperature on the activity of the enzyme trypsin? Thank you for your help

Answer 1

Trypsin is a digestive enzyme - it is found in the human gastrointestinal tract, which is 37 C in normal humans. It would be reasonable to wonder if it works best - i.e., has the highest catalytic capacity - at that temperature. So a very crude experiment would be to purify some trypsin and test its catalytic ability over a range of different temperatures.

More subtle approaches are possible - for example, the active from of trypsin is actually cleaved from a larger protein precursor. For trypsin to catalyse anything at all, it has to be activated by having the in-activating part of the protein removed. So you might look at temperature on the activity of the enzyme responsible for cleaving off the needless domains...

Answer 2

@blues Thank you very much for your prompt and informative answer. It sure did help a lot :)

Answer 3

Glad to be helpful. ;)