Question

Enzymekinetics exercise: I have a table of with a column of measured [S] (concentration of substrate Micrococcus lysodeikticus, mg/ml), and two columns with measured reactionspeeds (mg/ml min) for natural lyzosome (v[A]) and 50% denaturalized lyzosome (v[B]). I have to use this data to find v_max and Km for both cases. I'm thinking I must use the v=v_max[S]/K_m formula, but then I only have a ratio between v_max and K_m rather than the two separate identities. Or should I plot it and see that way what v_max and K_m is?

Answer 1

can you use excel, or does it have to be an analytical solution? A reciprocal plot \(\dfrac{1}v{}~vs~\dfrac{1}{[S]}\) would be the easiest.

Answer 2

It didn't say, but eventually that's what I did: a lineweaver-burke plot. And 1/Km were approximately the same, but 1/vmax altered. Although there wasn't inhibition going on, I could make conclusions on the ability of each type how they deal with inhibition.

Answer 3

good stuff!