Question

Urgent Help!!!!

Answer 1

whats the doubt my friend..!!

Answer 2

I dont know what sequence to input

Answer 3

ohh

Answer 4

ill try to help you okay..!

Answer 5

okay :)

Answer 6

@paki

Answer 7

@ganeshie8

Answer 8

@Abhisar please have a look here...

Answer 9

I am ryt now busy some wr else...I am sorry i can help but it will take time

Answer 10

okay, whenever possible

Answer 11

Using dideoxyribonucleotide triphosphates (ddNTPs) is a way to stop a strand from extending because the '3 -OH is not present, so there's nowhere to attach the next nucleotide because the extension is only in the 5' to 3' direction.

So you have the primer, it's complimentary to the strand on the bottom:

3' - GTGCAATAAT...CC-5'
5' - CACG - 3' -> -> -> (direction of extension)

Ideally, you will have incorporated a ddNTP at every point, so your fragments will differ by 1 base in length.

fragment 1: 5' - CACGT - 3'
fragment 2: 5' - CACGTT - 3'
fragment 3: 5' - CACGTTA - 3'

The fragments will migrate on the gel depending on their size ONLY because the distribution of charge along the strand is uniform (negative charge on the phosphates). So the gel functions as a sieving matrix, a smaller the fragment will migrate further from the origin (wells) than a longer one because of it.