Question

ststement 1:-restriction enzyme cuts down viral dna but does not harm bacterial chromosome

statement 2:-the plasmid in bacteria is cut with the same restriction enzyme which is used for isolation of gene of interest

first statement says it cant harm bacterial chromosome second says it is used to cut ..

any 1 can elaborate it please

Answer 1

@Preetha

Answer 2

@ankit042 @ganeshie8 @Gabylovesyou

Answer 3

@jhonyy9

Answer 4

i need a quick help any 1 plz

Answer 5

@Abhisar

Answer 6

Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells)

Answer 7

but bacterial DNA is protected from digestion because it is methylated and sometimes the restriction site needed is not present in chromosomal DNA

Answer 8

in molecular bio experiments, the principle is the same -- the methylation pattern in plasmid DNA is different and no more protects from restriction enzyme digestion..
and also the restriction enzymes we use in expts are from various different source of bacteria...like Bam H1, Bsa 1..

Answer 9

restriction endonucleases are used to cleave the bacterial DNA [extra nuclear genome - plasmid] and incorporate the foreign [desired gene - gene of interest] to produce transgenic plants, or to extract secondary metabolites etc..

Answer 10

the restriction endonuleases cuts the extranuclear genome of the bacteria but not the nuclear genome [nucleiod].

Answer 11

extranuclear genome is the circular double stranded genetic material which is only used in the reproduction process of the bacteira where we use that material for producing certain disease resistant plants.

the nuclear genome [nucleoid] is helpful for all the metabolic activities..!!

Answer 12

hey..@saroash ...got it now..?

Answer 13

yup :) em happy .. u made it so clear :) @BIOCRYSTAL thank u so much

Answer 14

@deepika.comet thanks to u too ... it was v helpful

Answer 15

:)

Answer 16

and most of the bacterial strains like E.coli we use in labs are engineered that they no longer have this restriction enzymes and hence can not cut our plasmids when they are inside them

Answer 17

i got it genious! :)

Answer 18

thanks for you too..i recollected them after a long time..
nice time

Answer 19

thnkoo

Answer 20

:)