Question

Can human DNA be used as a vector?

Answer 1

for making plasmids

Answer 2

what do you think plasmids are @Purplerainbowcherry

Answer 3

Plasmid is a small double stranded cicular DNA material which is physically separate from chromosomal DNA and has a capacity to replicate independently..

Do you think the Human DNA can be really made a plasmid with the above characters..!!

Answer 4

Human DNA can harvest a viral DNA making it a vector for infections.

Usually the virus would be a Retrovirus like HIV.

Answer 5

I think Humans have plasmid-like structures called Transposon elements.

They are segments of the DNA which can duplicate itself without making a copy of the whole DNA.

Answer 6

@oOKawaiiOo

but do they [transposon elements] function like plasmids when incorporated with a required foreign gene..??

Answer 7

@deepika.comet No you can't really compare transposable elements to plasmids. It would be more accurate to call them jumping genes.

It would not surprise me you could in fact make plasmid DNA from human DNA, but the process is very complicated (perhaps so much it is not wroth it)
First of all you need to isolate the DNA we are interested in, second we are interested in a uncoil it all from super negatively coiled to open right conformation we need. Last we gotta sequence everything to see if the isolated DNA contains the elements required for an good vector

Answer 8

worth it*

Answer 9

Compaired to for example e.coli this is highly complicated. Takes me less than a day to isolate genes from e.coli

Answer 10

You may need to do some genetic engineering if the sequence of the vector does not live up to the requirements of the vector.

Answer 11

I suggest you do a BLAST search against the DNA sequence you do and allowing, or you can try put in the gene you want on the vector first, then do a BLAST vs DNA sequence with open reading frames.

Answer 12

im still thinking.......

Answer 13

My only thought on DNA acting as a vector is the virus lysogenic pathway.

Answer 14

I just gotta ask: We are talking about plasmid vectors right? :P

Answer 15

Plasmid vectors are found in prokaryotes..... not human

Answer 16

Human have something else......i forgot the term...... but its similar to plasmids

Answer 17

super hypothetical: As we do not know what we want the vector be used for, we could isolate DNA from human, make insertions of relevant sequences for vector elements depending on the we want to preform a transformation on, and at the same time spend a lot of money for what we else could isolate from a bacteria?

Answer 18

But usually we use human artificial chromosomes for humans, but they are also considered vectors in some literature.

Answer 19

Vectors but not plasmids yes :)

Answer 20

In conclusion, yes, humans DNA can be used as a vector.

Is it beneficial? Process can be expensive and time consuming. So probably not the best method.

Answer 21

Indeed, you can make a vector with gene insertion in less than a day.

Answer 22

was that me who compared plasmids with human jumping genes @Frostbite

Answer 23

it was @oOKawaiiOo who compared them

Answer 24

and one more thing as giving direct answers is offence so i just wanted to give him a clue..
if i was wrong there you can specify me...

Answer 25

Well then lets get rid of the obvious then since we are so in detail now:
"Plasmid is a small double stranded cicular DNA material"
Wrong, they don't have to be double standard circular, they can take way more topologies to be functional - Sinkunas (2013) & Westa (2012)


The kind of reply you choose using isn't very accurate but simply question Kawaii, leaving it open for discussion.

Answer 26

ohh but i learnt plasmid to be double stranded circular ...

Answer 27

Learnt? from what? A high school book?

Answer 28

This is peer-reviewed research.

Answer 29

This perfectly lead to the next part:
Giving answers vs. clues.

This question can be made rather complicated, so having a health discussion next to also having an answer you give is only a great addition.

Answer 30

hey now what you actually wanted to tell

i didnt comfirm anything i wanted the person whoasked the question to try it on his own
what i did is just gave the definition of plasmid and left the thing with him..

Answer 31

^^ *confirm

Answer 32

i was just waiting his reply

Answer 33

But truly my responds about transposable elements (I hate the jumping gene term so popular science like :P), was not directed to you, it was mostly that the reply was left as a question so I choose to back up in that it can not be compared as a vector plasmid. :)

But truly, I think he most optimal is as you very well did: ask him what a plasmid is THEN you can ask your self "Can this actually be done?" which is a harder question

Answer 34

I just think I jumped straight to the discussion of the second question, instead of awaiting an answer from your clue to him.

Answer 35

ya i waited for an hour for his reply after my reply - i felt i gave him a clue..

im sorry if i was wrong..and rude to you..!!
:)

Answer 36

And you sure did, sorry I kinda ruined the clue.