B-Galactosidase is a hydrolase enzyme that catalyzes the hydrolysis of B-galactosides into monosaccharides. A .799 gram sample of B-galactosidase is dissolved water to make .193L of solution, and the osmotic pressure of the solution at 25 (Celsius) is found to be .882 mbar. Calculate the molecular mass of B-galactosidase. Please help I'm so lost on this question.

Question

aaronq · Answer

Osmotic pressure ($\Pi$) is given by the equation:  $\large \Pi=iMRT$

where $i$ is the van't hoff constant, M is molarity, R is the gas constant, and T is the absolute temperature.

Find the molarity of the solution, get the moles, then the molecular mass.

$\sf Molarity=\dfrac{moles~of solute}{L~of ~solution}$


$\sf moles=\dfrac{mass ~(in~g)}{Molecular~ mass}$