how do you find the detection limit?

Question

kkutie7 · Answer

@Jesstho.-. do you think you can help me?

kkutie7 · Answer

I know i don't have actual values, but I don't know what values I need

kkutie7 · Answer

here is the exact question 
1.	The detection limit for a given analysis is defined as the concentration of the analyte that would yield a signal approximately three times the noise level of the measurement. Estimate the detection limit for luminometric analysis of Tb3+ that is chelated to DTPA-pAS.

anonymous · Answer

@Kainui

kainui · Answer

Ok, so one way to do this is to collect several different measurements at different concentrations and create a line using the Beer-Lambert law to help you predict what you'd like.

kkutie7 · Answer

ok I've done that

kainui · Answer

So where does the noise fall on about average I guess? Multiply that by 3, and that's your detection limit it sounds like.

kkutie7 · Answer

well that where I'm confused because I have a mess of data and I don't exactly know what the noise is in my data.... I have a curve and got the concentration from it but that is as far as I've got

kainui · Answer

The noise is the part that's just sort of at the bottom, like this: |dw:1412832998457:dw|