Question

Having trouble understanding few slides about Enzymes

Answer 1

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Answer 2

@aaronq @abb0t @nincompoop

Answer 3

I dont exactly get what it says #_# can any of you clear it out, please?

Answer 4

What slide are you exactly confused about?

Answer 5

Can you be more specific?

Answer 6

What it is exactly saying about transition state

Answer 7

Well, first of all, I want to clear this common misconception about enzymes or catalysts.

They do NOT lower the Energy of activation of a particular species. They simply find an alternate route with a less expensive energy.

Answer 8

Yeah, i do know that

Answer 9

Do you know what transition state means?

Answer 10

yeah
its like middle state before product formation

Answer 11

its like intermediate

Answer 12

the transition state is the transition of molecular structure in which a molecule is no longer a substrate but not yet a product.

Answer 13

mhm mhm
from reading this
does that mean that its not exactly ' lock and key ' relationship between substrate & enzyme?

Answer 14

For some enzyme to make a rxn proceed faster, it actually is due to the fact that it stabilizes this transiton states energy, or as you know, activation energy, which is the minimum amount of energy required to break bonds of the reactants to form products.

Answer 15

okay

Answer 16

It locks to the binding site (active site)

Answer 17

so does that mean that enzyme
once substrate gets into active site
it is converted to its transition state
& if transition state can fit into it
they u can get the product?

Answer 18

I think i know what you're talking about when you say lock and key...
in this case, the lock would be the enzyme, and the subtrate, well, the key.
Only the right key (subtrate) fits into the active size of the lock (enzyme)

If the key wont fit, t wont locko correctly, and so forth.

Answer 19

The lock and key model is not the whole story, the combination of the former and the "induced fit" model is a better representation of reality because it takes into into account the flexibility of the reactant and the enzyme.

Answer 20

yeah
i've some about that
that active site is not exactly of substrates shape but a bit more flexible

Answer 21

Although, experimental data has shown that it doesn't always follow that key-lock analogy, but it is a good model.

Answer 22

two things can bind, but won't function, for example.

Answer 23

my confusion here is that
is lock and key concept related to
to be exact
enzyme = lock
and ' transition state ' of substrate = key

Answer 24

won't function meaning no product? as in competitive inhibition?

Answer 25

key = subtrate
fits into the key hole (active site)
lock = enzyme.

Answer 26

well, i mean they won't react.

Answer 27

im sure ou've had a key that goes in, but wont open the door.

Answer 28

yeah lol

Answer 29

ah! wait
so transition state! is a route chosen by enzyme for its catalysis?

Answer 30

did i get it???

Answer 31

For example Im sure you're fmailiar with Sn\(_2\) reaction from o-chem?

Answer 32

yeah yeah

Answer 33

breaking & bond formation is done in one step
so transition/ intermediate state has partially broken and partially formed bonds

Answer 34

im just stuck in 3rd slide to get to final conclusion

Answer 35

Yes. That mid rectangle section is the tranition state.

Answer 36

got that

Answer 37

Its the max energy, has partial bonds.

Answer 38

same with an enzyme. this is what is happening.

Answer 39

Don't over think it. It's quite simple, unless you get into enzyme kinetics, where it gets very complex, and involving a lot of math.

Answer 40

so enzyme receives the substrate at active side, converts it to transition state which fits it better & then changes it to final product
did i get it?

Answer 41

yes.

Answer 42

im trying to find a video for you, because for this, visuals are far better than simple verbal explantion.

It's like trying to explain this without that drawing.

Answer 43

yeah well just making sure im getting (coz i cant remember if i dont understand)
&
Thanks a bunch! All clear now :3
I already visualized it more or less :D

Answer 44

This might help.
https://www.youtube.com/watch?v=Dq0RUc8KNZk

Answer 45

thanks! i'll check it put
btw we are doing enzyme kinetics too
that formula thingy is creepy
gonna take time to get it

Answer 46

@aaronq too, thank you :)

Answer 47

michaelis menten?

Answer 48

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i guess so

Answer 49

oh video is great! http://prntscr.com/6bj4t3