If an anion exchange column (column is positively charged) was used in a neutral buffer, assign each amino acid to the corresponding peak in the chromatogram.

Leucine, Aspartate, Lysine and Threonine.

Question

If an anion exchange column (column is positively charged) was used in a neutral buffer, assign each amino acid to the corresponding peak in the chromatogram.

Leucine, Aspartate, Lysine and Threonine.

anonymous · Answer

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aaronq · Answer

Determine the strength of interaction of the amino acids with the column (focus on the R group). Cations will be eluted first and anions last.

anonymous · Answer

@aaronq Thanks for answering. I've never really dealt with chromatography before, let alone ion exchange chromatography. Is the R group the grey area? If it is, the more negative the amino acid's R group is, the faster it will elute?

aaronq · Answer

Yes, the grey area is the R group, and no the more negative the R group is the later/slower it will elute because of it's favourable interaction with the stationary phase (higher retention).

anonymous · Answer

@aaronq So, how high the peaks on the graph are have no bearing? I'm only focusing on how fast each amino acid eluted based on the charge of it's R group? If so, Lysine is A, Aspartate is B, Threonine is C and Leucine is D?

aaronq · Answer

the peak height would be correspondent to the concentration of each amino acid, the time/order of elution is what dictates what comes out first.

I think it's A is Lys, B is Leu, C is Thr and D is Asp

anonymous · Answer

Alright. Thanks alot.