Question

Will Medal!
(The question is one question)
How does the spectrometer work? What does the absorption of light waves help you to determine? How did you use the calibration curve to determine the blood alcohol content?

Answer 1

@YouSir
@AliSurfCat

Answer 2

im in chem love
lol sorry

Answer 3

Sorry me to but let me see who will help

Answer 4

@Compassionate @Jamierox4ev3r

Answer 5

It's Forensic Science.

Answer 6

@Danni_joanaveil

Answer 7

Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is rotated so that only a specific wavelength of light reaches

Answer 8

If it absorbs more light it is bad.

Answer 9

Thanks guys!

Answer 10

If you pass white light through a coloured substance, some of the light gets absorbed. A solution containing hydrated copper(II) ions, for example, looks pale blue because the solution absorbs light from the red end of the spectrum. The remaining wavelengths in the light combine in the eye and brain to give the appearance of cyan (pale blue).

Some colourless substances also absorb light - but in the ultra-violet region. Since we can't see UV light, we don't notice this absorption.

Different substances absorb different wavelengths of light, and this can be used to help to identify the substance - the presence of particular metal ions, for example, or of particular functional groups in organic compounds.

The amount of absorption is also dependent on the concentration of the substance if it is in solution. Measurement of the amount of absorption can be used to find concentrations of very dilute solutions.

An absorption spectrometer measures the way that the light absorbed by a compound varies across the UV and visible spectrum.



A simple double beam spectrometer

We'll start with the full diagram, and then explain exactly what is going on at each stage.

Answer 11

This may seem odd to someone not familiar with chemistry, since we all know that alcohol is a
clear colorless liquid. How can alcohol levels be determined using an instrument which is
sensitive to color? In order to use this method, the alcohol present in the suspect’s breath, blood
or in a beverage reacts with an orange-yellow chemical called potassium dichromate (K2Cr2O7).
When the alcohol reacts with the potassium dichromate, a blue-green compound called
chromium(III) sulfate is produced. The reaction is as follows:
2K2Cr2O7 + 8H2SO4 + 3CH3CH2OH → 2Cr2(SO4)3 + 2K2SO4 + 3CH3COOH + 11 H2O
orange-yellow alcohol blue-green
The spectrophotometer is set to absorb the blue-green (560 nm) color of light that is produced in
the reaction. Using the above reaction, it is safe to say that the more blue-green light that is
absorbed by the spectrophotometer, the more alcohol present in the sample.

Answer 12

I'm in 9th grade so obviosuly I haven't reached to Chemisty yet.