Question

If you received an mystery transposon on a plasmid, what sort of “marker” could you insert into this transposon to determine if it is a DNA transposon or a retrotransposon

Answer 1

DNA Transposons, which move within a genome by means of a DNA intermediate
Retrotransposons, which move by means of an RNA intermediate

In Retrotransposition, Reverse Transcriptase makes copy of DNA by Retrotransposon RNA.

So if we induce Reverse-transcriptase inhibitors (RTIs) in the plasmid sample & it doesn't show transposition its Retrotransposons otherwise its DNA Transposons

Answer 2

Transposons are segments of DNA that can move around to different positions in the genome of a single cell.
There are two distinct types:
Class II transposons. These consist of DNA that moves directly from place to place.
Class I transposons. These are retrotransposons that first transcribe the DNA into RNA and then use reverse transcriptase to make a DNA copy of the RNA to insert in a new location.

Answer 3

I agree with u @RamiroCruzo