Will infectious virus be produced if the genome of an enveloped (+) strand RNA virus is added to an extract prepared from the cytoplasm of eurokaryotic cells that retains translational activity but lacks DNA replication or transcription of host genes?

Question

photon336 · Answer

@somy

somy · Answer

No
and the reason is something you know of, thats why you will answer it by answering my questions
1 - what is the aim of the retrovirus that enters a host cell?
2 - how does DNA replication happen? (what do u need here)
3 - how does transcription happen & what is the final product that will enter translation phase? (and what do u need here)

somy · Answer

full answer to your question is in basic principals of these activities, thats why lets walk through it first

somy · Answer

or, i believe you do know the entire process so if you want to go ahead and start analysis, watch this https://www.youtube.com/watch?v=HhhRQ4t95OI and think of the conditions provided, then tell me what do you think is the issue here?

somy · Answer

conditions by your question i mean

kainui · Answer

That video was awesome. :O

photon336 · Answer

Yeah thanks for the video, @Somy like the HIV basically integrates into the host DNA and is replicated along with the host DNA. so it would most likely follow a lysogenic cycle?

somy · Answer

well thats one type of retrovirus

somy · Answer

now the issue here is the fact that we cant do transcription of that RNA

somy · Answer

and so we dont have mRNA of it thats why we cant translate it

somy · Answer

because thats the only system left in that cell having just cytoplasm with it

photon336 · Answer

Alright, so say we've got a (-) strand RNA virus. 

so it needs to carry its own RNA polymerases, 

so the (-) means that it has a strand that is complimentary to the to one we need to replicate. 

so, my book is like okay, the - strand can't really create any functional protein if translated. 


but if' that strand is complimentary then why cant it produce functional protein, isn't all the genetic material  already in the (-) strand? 

is it because RNA is naturally single stranded?

somy · Answer

& there is another thing, its not a cell 
u know how usually the envelope fuses with the membrane? here there is no membrane mentioned in the q

photon336 · Answer

okay so technically since there is no envelope, the virus can't insert it's genetic material into the cell? that step is skipped.