a ) Assuming 58.2% efficiency, how much free energy would be liberated by the flow of
protons down their gradient from intermembrane space to matrix given that: temperature is 37.2°C, there is a 0.168 mV transmembrane differential and a pH differential of 1.44?

b) At this transmembrane potential, the mitochondrian is said to be in a State IV condition
where the [ADP]/[ATP] ratio completely inverts such that [ADP] = 2.2 mM, [ATP] = 9.8 mM
and [Pi] = 2.3 mM. At this transmembrane potential the
laws of mass action with respect to this high-energy phosphate carrier do not favour its synthesis.Calculate the number of moles of ATP that can be synthesized by ATP synthase utilizing the energy you calculated in part a. (Use the provided concentrations and tabulated hydrolysis values
(excess Mg2+). Atp hydrolysis has a standard delta G of -30.5kj/mol.

Question

a ) Assuming 58.2% efficiency, how much free energy would be liberated by the flow of
protons down their gradient from intermembrane space to matrix given that: temperature is 37.2°C, there is a 0.168 mV transmembrane differential and a pH differential of 1.44?

b) At this transmembrane potential, the mitochondrian is said to be in a State IV condition
where the [ADP]/[ATP] ratio completely inverts such that [ADP] = 2.2 mM, [ATP] = 9.8 mM
and [Pi] = 2.3 mM. At this transmembrane potential the
laws of mass action with respect to this high-energy phosphate carrier do not favour its synthesis.Calculate the number of moles of ATP that can be synthesized by ATP synthase utilizing the energy you calculated in part a. (Use the provided concentrations and tabulated hydrolysis values
(excess Mg2+). Atp hydrolysis has a standard delta G of -30.5kj/mol.

winterdays23 · Answer

For part a) i got -4.97 kj/mol